Burden of Dengue Virus Detection by Serological and Molecular Method

  • Yogendra Shah Everest International Clinic and Research Center, Kathmandu, Nepal
  • Govind P. Gupta Everest International Clinic and Research Center, Kathmandu, Nepal
  • Dharam P. Kuikel Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal
  • Krishna P. Pant Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal
  • Ganesh Khadka Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal
  • Biswas Neupane Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal
  • Gyan B. Aryal Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal
  • Kishor Pandey Everest International Clinic and Research Center, Kathmandu, Nepal
  • Basudev Pandey Everest International Clinic and Research Center, Kathmandu, Nepal
Keywords: Dengue, IgM capture, ELISA, RT-PCR

Abstract

Dengue is an emerging mosquito-borne viral disease in the world and is the serious public heath problem of Nepal. This study was designed to determine sero-epidemiology of dengue virus infection among suspected patients with fever during June-November 2010 visiting Koshi Zonal Hospital (KZH), Biratnagar; Narayani sub-regional Hospital (NSH), Birgunj; Sukraraj Tropical and Infectious Disease Hospital (STIDH), Kathmandu and Dhading District Hospital (DDH), Dhadingbeshi. The seroprevalence of anti-dengue IgM antibody was determined by enzyme linked immunosorbent assay (ELISA). Among 271 serum samples tested, the anti-dengue IgM positivity was 14.4%. Sero-positivity in male was 10.7% of total and that in female was 3.7%. Among different age groups, the highest positive cases 11.8% were from age group 15-50 years and found least among the age group above 50 years 0.4%. Out of four different hospitals, the highest positive cases (9.2%) from STIDH and the least positive cases (0.4%) were from DDH. Molecular diagnosis based on RT-PCR was also performed for the confirmatory diagnosis in the acute IgM ELISA negative samples and out of 21 samples tested, one sample was found to be 4.7% positive for dengue consensus.

Published
2012-12-10
How to Cite
Shah, Y., Gupta, G. P., Kuikel, D. P., Pant, K. P., Khadka, G., Neupane, B., Aryal, G. B., Pandey, K., & Pandey, B. (2012). Burden of Dengue Virus Detection by Serological and Molecular Method. The Journal of University Grants Commission, 1(1), 83-91. Retrieved from http://jugc.edu.np/index.php/JUGC/article/view/113